首页|A colorimetric nanobiosensor with enhanced sensitivity for detection of lead(Ⅱ)in real-water samples via an adenine-cytosine mismatched DNAzyme
A colorimetric nanobiosensor with enhanced sensitivity for detection of lead(Ⅱ)in real-water samples via an adenine-cytosine mismatched DNAzyme
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
万方数据
Facile and ultrasensitive detection of Pb2+in water for remote or resource-limited environments remains challenging.DNAzyme-based colorimetric nanobiosensors have been extensively studied to regulate the assembly of functionalized gold nanoparticles(AuNPs).However,these nanobiosensors have been criticized for their low sensitivity owing to the difficulty of dissociating DNAzyme embedded in AuNP aggregates.To address this issue,we rationally designed a DNAzyme by introducing an adenine-cytosine(A-C)mismatch to strengthen the disassembly of DNAzyme-linked nanostructures.As proof of concept,a"turn on"colorimetric nanobiosensor integrated with mismatched DNAzyme and functionalized AuNPs was first developed for Pb2+detection.Under the optimal detection conditions,the obtained typical calibration curve shows a detection limit of 8.6 nmol/L,with an approximately 11-fold sensitivity improvement in Pb2+detection compared with unmismatched DNAzyme,and a linear response range from 10 to 300 nmol/L.This nanobiosensor demonstrated robust selectivity and satisfactory recovery rates between 86.5％and 106.4％for Pb2+in spiked environmental water samples.Additionally,the detection process is user-friendly and can be completed within 30 min,requiring only a simple water sample addition step.Considering the extensive applications of DNAzyme in conjunction with nanoparticles,this study provides a valuable reference for designing other DNAzyme-powered nanoparticle assemblies in biosensing systems.
Trace lead(Ⅱ)ionsModified mismatched DNAzymeGold nanoparticlesColorimetric nanobiosensorEnvironmental water samples
万方数据
