Abstract
A water-soluble polysaccharide (CPS-0) was obtained from the root of Cudrania tricuspidata (Carr.)Bur.,by hot water extraction (70℃),deproteination using enzymolysis and Sevag method,precipitation with ethanol,and fractionation through DEAE-Sephadex A-50 chromatography.The purity of CPS-0 was determined by HPLC and the structure was elucidated by monosaccharide composition analysis,methylation analysis,GC,GC-MS,NMR spectral (1H-NMR,13C-NMR,HMQC),UV,IR,and elemental analysis.The CPS-0 was found to contain glucose residues only.The average repeating unit is a decasaccharide having a backbone consisting of 1,41inked α-D-glucopyranosyl residues to which the side chain consisting of terminal and 1,4-1inked α-D-glucopyranosyl residues was attached at position 6 of the branching residues.
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