首页|Dendrimer-induced synthesis of porous organosilica capsules for enzyme encapsulation
Dendrimer-induced synthesis of porous organosilica capsules for enzyme encapsulation
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
NETL
NSTL
万方数据
Organic matter-induced mineralization is a green and versatile method for synthesizing hybrid nanostructured materials,where the material properties are mainly influenced by the species of natural biomolecules,linear synthetic polymer,or small molecules,limiting their diversity.Herein,we adopted dendrimer poly(amidoamine)(PAMAM)as the inducer to synthesize organosilica-PAMAM network(OSPN)capsules for mannose isomerase(MIase)encapsulation based on a hard-templating method.The structure of OSPN capsules can be precisely regulated by adjusting the molecular weight and concentration of PAMAM,thereby demonstrating a substantial impact on the kinetic behavior of the MIase@OSPN system.The MIase@OSPN system was used for catalytic production of mannose from D-fructose.A mannose yield of 22.24％was obtained,which is higher than that of MIase in organosilica network capsules and similar to that of the free enzyme.The overall catalytic efficiency(kcat/Km)fructosewof the MIase@OSPN system for the substrate D-fructose was up to 0.556 s-1·mmol-1·L.Meanwhile,the MIase@OSPN system showed excellent stability and recyclability,maintaining more than 50％of the yield even after 12 cycles.
School of Environmental Science & Engineering,Tianjin University,Tianjin 300072,China
Collaborative Innovation Center of Chemical Science and Engineering,Tianjin 300072,China
State Key Laboratory of Biochemical Engineering,Institute of Process Engineering,Chinese Academy of Sciences,Beijing 100190,China
Key Laboratory for Green Chemical Technology of Ministry of Education,School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China
展开 >
国家重点研发计划国家重点研发计划Tianjin Synthetic Biotechnology Innovation Capacity Improvement ProjectOpen Funding Project of the State Key Laboratory of Biochemical Engineering of ChinaHaihe Laboratory of Sustainable Chemical Transformations
NETL
NSTL
万方数据
