Abstract
The random amplified polymorphic DNA (RAPD) technique was used to evaluate the genetic diversity and population structure of 91 genets from four wild populations of Betula luminifera at different eleva-tions in the National Nature Reserve of the Wuyi Mountain,Fujian Province, China. Eighteen random primers (from 139 primers) produced a total of 199 scorable amplified fragments, of which 174 (87.44%) were polymorphic across all individuals. The genetic diversities of B.luminifera at the population level and species level were PPL = 60.05%, h = 0.2242, I= 0.3181 and PPL = 87.44%,h = 0.3442, I= 0.4899, respectively. The value of differ-entiation (Gst= 0.3486) and analysis of molecular variance (AMOVA) indicated that there was a relatively high genetic differentiation among populations, and about one-third of the genetic variation occurred among populations. Pearson correlation analysis further revealed that the genetic diversity within populations had significant or very significant correlation with the elevation, climatic factors (annual average temperature and annual precipitation) and soil nutrient factors (total nitrogen, C/N ratio and organic matter). Mantel tests show that there was a significant correlation between the genetic distances among popula-tions and the distance of elevation, and the divergence of soil nutrient factors. The results of the present study suggested that the relatively high genetic differentiation among populations ofB. luminifera at different elevations might be caused by ecological factors and gene flow.
基金项目
Southern Mountain Timber Forest Cultivation Laboratory of Forestry Ministry of China()
NETL
NSTL
万方数据