首页|RAPD markers related to sex locus in Populus tomentosa

RAPD markers related to sex locus in Populus tomentosa

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By using the methods of random amplified polymorphic DNA (RAPD) and bulked segregate analysis (BSA), we identified markers that are linked to the sex determination in the dioecious Populus tomentosa. Male and female bulks were created through rough mixing equal amounts of its five individual DNA. A total of 88 primers were screened. Twelve primers produced clear patterns with at least one band that appeared to be polymorphic between the two bulks. Subsequently, five male and female individuals were analyzed with those 12 primers, and only S60 (ACCCGGTCAC) could generate a common 1800bp DNA fragment in all five male individuals and male pool but not in any female individuals. It can be concluded that the gender of P. tomentosa is most likely connected to the S60-1800bp DNA fragment and RAPD markers. S60, therefore, can be used for selecting the gender of P. tomentosa.

biotechnologyRAPDbulked segregate analysis (BSA)Populus tomentosa

Wanwei HOU、Junfeng FAN、Feimei ZHOU、Shufang ZHAO

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School of Forestry, Northwest Agriculture & Forestry University, Yangling 712100, China

Project in the National Science & Technology Pillar Program during the Eleventh Five-Year Plan Period

2006BAD01A1502-2

2009

10.1007/s11416-009-0037-z

中国高等学校学术文摘·林学
高等教育出版社

中国高等学校学术文摘·林学

ISSN：1673-3517
年,卷(期)：2009.4(2)
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