Abstract
ITS sequences of ten kinds of plants of Lespedeza and an out group were obtained by primer design PCR, sequencing and cluster analysis. The results show that ITS1 section length was 228-243 bp, 5.8S sequence length was 165 bp which was very conservative; ITS2 section lengths varied from 215 to 220 bp and the conservative sites occupied 88.1%. Phylogenetic analysis of ITS sequences using PAUP 4.0 software and their genetic relationship were discussed.
基金项目
Chinese National Programs for High Technology Research and Development(2002AA241111)
Nomarch Special Fund for Excellent Talent of Science and Technology Education of Guizhou Province(Z073224)