中国高等学校学术文摘·林学2009,Vol.4Issue(4) :489-493.DOI:10.1007/s11461-009-0069-4

Phylogenetic relationship of genus Lespedeza by ITS sequence data

Xiurong WANG Yang ZHAO Ruiqi PIAN Xiaoyang CHEN
中国高等学校学术文摘·林学2009,Vol.4Issue(4) :489-493.DOI:10.1007/s11461-009-0069-4

Phylogenetic relationship of genus Lespedeza by ITS sequence data

Xiurong WANG 1Yang ZHAO 1Ruiqi PIAN 2Xiaoyang CHEN3
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作者信息

  • 1. Forestry College of Guizhou University, Guiyang 550225, China
  • 2. College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China
  • 3. South China Agricultural University, Guangzhou 510642, China
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Abstract

ITS sequences of ten kinds of plants of Lespedeza and an out group were obtained by primer design PCR, sequencing and cluster analysis. The results show that ITS1 section length was 228-243 bp, 5.8S sequence length was 165 bp which was very conservative; ITS2 section lengths varied from 215 to 220 bp and the conservative sites occupied 88.1%. Phylogenetic analysis of ITS sequences using PAUP 4.0 software and their genetic relationship were discussed.

Key words

Lespedeza Michx/internal transcribed spacer (ITS)/phylogeny

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基金项目

Chinese National Programs for High Technology Research and Development(2002AA241111)

Nomarch Special Fund for Excellent Talent of Science and Technology Education of Guizhou Province(Z073224)

出版年

2009
中国高等学校学术文摘·林学
高等教育出版社

中国高等学校学术文摘·林学

ISSN:1673-3517
被引量1
参考文献量2
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