首页|Comparative karyotypic analysis of A and C genomes in the genus Oryza with Cot-1 DNA and RFLP

Comparative karyotypic analysis of A and C genomes in the genus Oryza with Cot-1 DNA and RFLP

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Fluorescence in situ hybridization (FISH) was applied to somatic chromosomes preparations of Oryza sativa L. (AA), O. Glaberrima (AA), and O. Officinalis Wall. (CC) with a labeled probe of C0t-1 DNA. Genomic in situ hybridization to its own chromosomes (self-GISH) was conducted in a control experiment. The homologous chromosomes showed similar signal bands probed by C0t-1 DNA, while karyotypic analysis of chromosomes between A genome in the two cultivated species and C genome in O. Officinalis were conducted based on the band patterns. The ideograms with C0t-1 DNA signal bands were also built. The nonuniform distribution of hybridization signals of C0t-1 DNA from O. Sativa and that on its own chromosome of O. Officinalis were observed. However, the similarity and correspondence between C0t-1 DNA signal patterns and genomic DNA signal patterns indicated that the self-GISH signals actually resulted from the hybridization of genomic repetitive sequences to the chromosomes. The restriction fragment length polymorphism (RFLP) marker, R2676, from the chromosome 8 of O. Sativa and O. Officinalis, was used as a probe to somatic hybrid on chromosomes for comparative karyotypic analysis between O. Glaberrina and O. Officinalis. The results showed that R2676 was located on the short arm of chromosome 7 in O. Officinalis and chromosome 4 in O. Glaberrima. The percentage distances from the centromere to hybridization sites were 91.56±-5.62 and 86.20±3.17. Our results revealed that the relative length of O. Officinalis chromosome 8 does not follow conventional chromosome length in descending order of number. C0t-1 DNA of A genome signals were detected in the end of the short arm of O. Officinalis chromosome 8, indicating that the highly and moderately repetitive DNA sequences in this region were considerably similar between C and A genomes. However, the fluorescence intensity on the chromosomes of C0t-1 DNA ofA genome was less than that of its own C genome from O. O~cinalis, which would be one of the causes for the fact that highly and moderately repetitive DNA sequences were amplified in O. Officinalis. No homology signal of C0t-1 DNA from O. Sativa was detected in the end of the long arm of O. Glaberrima, indicating that repetitive DNA sequences of A genome in two cultivated rice were lost in the evolutional history. In this paper, using comparative karyotypic analysis of RFLP combined C0t-1 DNA signal bands, the evolutionary mechanism of genome in genus Oryza was also discussed.

C0t-1 DNARFLPkaryotypein situ hybridizationOryza

Junbo ZHOU、Weizhen LAN、Rui QIN

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College of Life Sciences,Huzhou Teachers College,Huzhou 313000,China

The Third Middle School of Wuyi,Jinhua 321200,China

Key Laboratory of State Ethnic Affairs Commission for Biological Technology,College of Life Sciences,South-Central University for Nationalities,Wuhan 430074,China

National Program of High Technology DevelopmentScientific Research Foundation for the Returned Overseas Chinese Scholars,Ministry of Education of the People's Republic of 中国博士后基金Project of Science and Technology for Youth,Wuhan,China

2004AA227120BZY04003200403505742004500607135

2011

10.1007/sl1703-011-1092-4

中国农学前沿
高等教育出版社

中国农学前沿

ISSN：1673-7334
年,卷(期)：2011.5(2)
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