首页|Development of EST-SSR markers in peanut (Arachis hypogaea L.)

Development of EST-SSR markers in peanut (Arachis hypogaea L.)

扫码查看
原文链接
  • NETL
  • NSTL
  • 万方数据
More molecular markers for potential use in peanut genetic research were developed.A total of 92403 EST sequences of peanut (Arachis) in the NCBI database were downloaded and analyzed.2594 SSRs distributed in 2267 nonredundant ESTsequences were detected,with tri-nucleotide motif (65.54％) as the most abundant motif type followed by di-nucleotide motif (28.10％).Among the 92 repeat types,the top eight motif types were AG/TC (20.1％),AAG/TTC (11.8％),AAT/TTA (10.1％),AGG/TCC (6.6％),AGA/TCT (6.3％),AT/TA (5.9％),ACT/TGA (3.8％),and ATG/TAC (3.7％) with higher frequency.A total of 237 primer pairs were successfully designed based on the 2267 SSR-ESTs using DNA star software.

peanutEST-SSRdevelopment

Xinyan WEI、Lifeng LIU、Shunli CUI、Huanying CHEN、Jingjing ZHANG

展开 >

Agricultural University of Hebei, Baoding 071001, China

This work was financially supported by the Natural Science Fund of Hebei Province,ChinaProject for the study of Application Foundation of Hebei Provincespecial fund for Modem Agro-industry Technology Research System,China

C200900059110960120D

2011

10.1007/sl1703-011-1091-5

中国农学前沿
高等教育出版社

中国农学前沿

ISSN：1673-7334
年,卷(期)：2011.5(3)
  • 2
  • 4