首页|Cloning and pharmaceutical analysis of CaMK gene of Botrytis cinerea
Cloning and pharmaceutical analysis of CaMK gene of Botrytis cinerea
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
NETL
NSTL
万方数据
Using a PCR homology approach,DNA and cDNA sequences of calcium/calmodulin-depcndent protein kinase (CaMK) gene ofBotrytis cinerea were obtained.Southern blotting result displayed that CaMK was single copy in the genome of B.cinerea.The cDNA sequence of CaMK revealed an open reading frame of 2190 nucleotides encoding a 730 amino acid protein with predicted molecular weight of 81.8748 kDa.The genomic sequence of CaMK revealed the same ORF interrupted by six introns.Bioinformatics analysis showed that this protein had the distinctive features that characterize CaMK ATP binding region signature and serine/threonine protein kinase active-site signature.Pharmaceutical analysis displayed that the CaMK specific inhibitor,KN-62,could inhibit conidial germination,pathogenicity and herbicidal activity ofB.cinerea BC4 strain.It was suggested that CaMK played an important role in regulating conidial germination,pathogenicity and herbicidal activity of B.cinerea.
NETL
NSTL
万方数据
