首页|Identification of a novel enhancin-like gene from Bacillus thuringiensis
Identification of a novel enhancin-like gene from Bacillus thuringiensis
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An enhancin-like gene was cloned from Bacillus thuringiensis (Bt) strain GS8 isolated from soil samples in china.The sequence analysis revealed that an open reading frame (ORF) of 2202 nucleotides encoding a protein containing 733 amino acids with a molecular mass of 84 kDa.The enhancin-like protein showed 100％ identity to Bel protein (FJ644935) and 23％-41％ identity to viral enhancin proteins; in the 252 to 261 amino-acid sequence of enhancin-like protein,a conserved metal binding motif (HEIAH) similar to that in the reported bacterial enhancin-like proteins was found (HEXXH in viral enhancin protein),which indicated that the enhancin-like protein belongs to metalloprotease.The purified enhancin-like protein was fed together with Cry9Ea to Spodopera exigua and Trichoplusia ni larvae,but no significant increase in toxicity was observed.
College of Life Sciences, Agricultural University of Hebei, Baoding 071001, China
College of Plant Protection, Agricultural University of Hebei, Biological Control Center of Plant Diseases and Plant Pests of Hebei Province, Baoding 071001, China
National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding 071001, China
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