首页|Screening of conidium development mutant of Botrytis cinerea and functional analysis of the related gene
Screening of conidium development mutant of Botrytis cinerea and functional analysis of the related gene
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A novel conidium development mutant was obtained by screening the transformants of Botrytis cinerea produced by Agrobacterium tumefaciens mediated method,which lost the ability of producing conidia.The flanking sequence of T-DNA insertion site was acquired by TAIL-PCR technology,and then,the T-DNA insertion in the second exon of BC1G_02800.1 confirmed by BLAST between the flanking sequence and the known sequence in the B.cinerea gene database.The mutant gene was identified as BC1G_02799.1 located in the upstream ofBC1G_02800.1 gene by RTPCR.The DNA full-length sequence of BC1G_02799.1 was 1951 bp and contained 1848 bp coding region,which encoded a 615 amino acids putative protein similar to ABC-transporter,and the function of BC1G_02799.1 gene was unknown to date.Phenotype analysis of the mutant found that the mutant strain colony was white,grew slowly,and did not produce conidium and sclerotia on PDA medium but showed a stronger pathogenicity to tomato leaves and successfully increased the enzyme activity related to pathogenicity compared to the wild type strain.The results suggested that the BC1G_02799.1 gene was involved in the conidium development,the sclerotia formation,and pathogenicity in B.cinerea.Our research will facilitate in understanding the molecular mechanism of conidium development,sclerotia formation,and pathogenic in B.cinerea.
Botrytis cinereaT-DNA insertional mutantTAIL-PCRconidium development
NETL
NSTL
万方数据
