首页|Construction of a Fosmid genomic library of Streptomyces roseoflavus Men-myco-93-63
Construction of a Fosmid genomic library of Streptomyces roseoflavus Men-myco-93-63
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To clone the antibiotic biosynthesis gene cluster ofStreptomyces roseoflavus Men-myco-93-63,we constructed a Fosmid genomic library.The genomic DNA of the strain Men-myco-93-63 was isolated by the modified CTAB procedure,and the size of most genomic DNA fragments was larger than 150 kb.Then,a Fosmid genomic library containing more than 6000 clones was constructed.The average size of the inserted DNA in recombinant plasmids was 38.1 kb,and the probability of harboring any gene in the genome of the strain Men-myco-93-63 was 99.99％.The library coverage was at least a 10-fold genome equivalent.Therefore,the constructed Fosmid library meets the requirements as a standard genomic library
Biological Control Center of Plant Diseases and Plant Pests of Hebei Province, College of Plant Protection, Agricultural University of Hebei, Baoding 071000, China
College of Life Sciences, Agricultural University of Hebei, Baoding 071000, China
National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding 071000, China
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