首页|Observation of the infection process of Metarhizium anisopliae on the cuticle of Anoplophora glabripennis larvae with scanning electron microscopy
Observation of the infection process of Metarhizium anisopliae on the cuticle of Anoplophora glabripennis larvae with scanning electron microscopy
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The invasion behavior and infection process of Metarhizium anisopliae on different cuticle areas of Anoplophora glabripennis larvae were observed by scanning electron microscopy.The results indicated that M.anisopliae infected A.glabripennis larvae mainly through the intersegmental membrane where more attaching conidia,faster germination,and higher germination and penetration rates were observed.The secondary invasion area was around the valve.The two areas were vulnerable to infection from M.anisopliae.Twelve hours after vaccination,conidia germinated and bud-shaped protrusions formed on the cuticle of A.glabripennis larvae,then germ tubes and various attaching structures were produced.Conidia penetrated the integument into the hemocoele ofA.glabripennis larvae 36- 48 h later.
Metarhizium anisopliaeAnoplophora glabripennisdifferent areas of cuticleinvasion behaviorinfection processscanning electron microscopy
Haixia YAN、Da WANG、Xusheng ZHAO、Dazhuang HUANG、Xiangchao MA
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Forestry College, Agricultural University of Hebei, Baoding 071000, China
Key Laboratory of Forest Germplasm and Protection of Hebei Province, Baoding 071000, China
Shanghai Academy of Agricultural Sciences, Shanghai 201106, China
International Cooperation Office, Agriculture University ofHebei, Baoding 071000, China
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