Seasonal dynamic changes in bacterial compositions in the Inner Mongolia desert steppe

扫码查看

原文链接

NETL
NSTL
万方数据

外文摘要：Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) technique was employed to examine the seasonal dynamic changes in bacterial community composition in the Inner Mongolia desert steppe using specific primers F954 and R1369.Bright and reproducible bands were sequenced,and the phylogenic tree was constructed.The results show that the bacterial community composition changed between different seasons.The specific bands were different between the sampling sites with light and heavy levels of degraded grassland.Three main types of bacteria constituting the microbial community in the Inner Mongolia desert steppe belonged to the α,γ and δ-sub phyla of Proteobacteria,Bacteroidetes and Acidobacteria.The unculturable bacteria accounted for 69% of the whole bacterial community of the Inner Mongolia desert steppe.

外文关键词：

Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE)Inner Mongolia desert steppecommunity compositionseasonal dynamic change

作者：

Ping LU、Wanli MA、Hao LI、Yongsheng WU、Guifen LV、Jizheng HE

展开 >

作者单位：

College of Life Science and Technology,Inner Mongolia Normal University,Huhhot 010022,China

Planning and Design Academy of Forestry Products Industry,State Forestry Administration,Beijing 100714,China

Research Center of Ecology and Environment,Chinese Academy of Sciences,Beijing 100083,China

基金：

国家自然科学基金University Scientific Research Foundation of Inner Mongolia

项目编号：

30560030NJ05099

出版年：

2009

DOI：

10.1007/s11515-009-0002-0

生物学前沿

高等教育出版社

生物学前沿

影响因子：0.2

ISSN：1674-7984

年,卷(期)：2009.4(2)

参考文献量1