首页|RNA-dependent pseudouridylation catalyzed by box H/ACA RNPs
RNA-dependent pseudouridylation catalyzed by box H/ACA RNPs
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BACKGROUND:Pseudouridine (Ψ) is the most abundant post-transcriptionally modified nucleotide found in RNA.Ψ is clustered in functionally important and evolutionary conserved regions of RNAs in all three domains of life.Pseudouridylation is catalyzed by two distinct mechanisms:an RNA-independent and an RNA-dependent mechanism.The former involves a group of stand-alone protein enzymes,and the latter involves a family of complex enzymes called box H/ACA RNPs,each of which consists of one RNA (box H/ACA RNA) and a set of four core proteins.Over the years,the mechanism of RNA-dependent pseudouridylation has been extensively studied.The crystal structures of partial and complete box H/ACA RNP have been solved.However,the detailed picture of RNA-dependent pseudouridylation is still not entirely clear.OBJECTIVE:In this work,we review what is known about box H/ACA RNP and the mechanism by which box H/ACA RNP catalyzes RNA-dependent pseudouridylation.We also discuss some examples of the dual nature and redundancy of box H/ ACA RNPs that deviate from the usual mechanism.METHODS:A methodical literature search was performed using the Pubmed central search engine and Intemational Digital Publishing Forum (EPUB) using the following keywords:"pseudouridylation," "pseudouridine," and "box H/ACA RNP." The necessary information was extracted and cited.RESULTS:A detailed introduction is made including the discovery,mechanism and crystal structure of box H/ACA RNP.Three sequence/structural requirements for box H/ACA RNA-guided pseudouridylation are discussed and the exceptions to those rules are explored.CONCLUSION:Over the years,box H/ACA RNP-catalyzed pseudouridylation has been extensively studied,generating fruitful results.However,a detailed picture regarding the mechanism of this reaction is still to be deciphered.More work is needed to fully understand box H/ACA RNP-catalyzed pseudouridylation.
Meemanage D.De Zoysa、Yi-Tao Yu
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University of Rochester Medical Center, Department of Biochemistry and Biophysics, Center for RNA Biology, 601 Elmwood Avenue, Rochester, NY14642, USA
work carried out in the Yu laboratory was supported from NIH (to Y-T Yu)
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