Abstract
BACKGROUND:The possibility of dietary ginger to enhance oxidative stress resistance and to extend life span was studied on Drosophila melanogaster.METHODS:Oxidative stress was induced by a reducing agent dithiothreitol.Experimental groups of male D.melanogaster were cultured on media containing:1) no additive;2) dithiothreitol,added into the nutritional mixture to the final concentration of 10 mM;3) 25 mg of ginger powder g-1 of the nutritional mixture;and 4) 10 mM ofdithiothreitol and 25 mg of ginger powder g-1 of the nutritional mixture.The number of alive fruit flies was inspected daily,and mean life span was determined for each experimental group.RESULTS:The addition of dithiothreitol to D.melanogaster nutritional mixture was established to result in an increase in concentration of two markers of oxidative stress conditions (thiobarbituric acid reactive substances as products of lipid peroxidation and carbonylated proteins as products of protein oxidation) in fly tissues.It was followed by significant reduction of mean life span and maximum life span of the last 10％ of flies.Plant preparation,being added simultaneously with dithiothreitol,significantly diminished the negative effects of this xenobiotic.In conditions of additional stress load induced by hydrogen peroxide or high temperature,survival of insects treated with dithiothreitol on the background of ginger powder was the highest.CONCLUSIONS:Thus,the presented data give the evidence that ginger preparations can reduce oxidative stress outcomes and significantly increase the life expectancy of fruit flies in stress conditions.
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NSTL
维普
万方数据