Design of miniature head-mounted fluorescence microscope based on metalens
The recent advent of miniature head-mounted fluorescence microscopes has revolutionized brain science research,enabling real-time imaging of neural activity in the brains of free-moving animals.However,the pursuit of miniaturization and reduced weight often results in a limited field of view,constrain-ing the number of neurons observable.While larger field-of-view systems exist,their increased weight can impede the natural behaviors of the subjects.Addressing these limitations,a novel design utilizing a metalens schematic is proposed.This approach offers the benefits of being ultra-light,ultra-thin,and capable of high-quality imaging.By deriving the aberration formula specific to hyperbolic phase metalens and using it as a foundation,a design for a miniature fluorescence microscope was developed.This microscope boasts a 4 mm×4 mm field of view and a numerical aperture(NA)of 0.14,effectively correcting seven primary aber-rations.The resulting prototype,weighing a mere 4.11 g,achieves a resolution of 7.8 μm across the entire field of view.This performance is sufficient to image neural activity in the brains of freely moving mice with single-cell resolution.
miniature fluorescence microscopemetalensoptical designneuron scienceaberration theory
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