Screening and identification of obesity-related genes in cervical ossification of posterior longitudinal ligament
Objective:To explore the differentially expressed obesity-related genes and their function in the cervical ossification of posterior longitudinal ligament(OPLL).Methods:The OPLL-related transcriptome dataset GSE69787 was downloaded from the Gene Expression Omnibus(GEO)database,and obesity-related genes were screened in the Gene database to obtain obesity-related differentially expressed genes(DEGs)in OPLL.Gene ontology(GO)and Kyoto encyclopedia of genes and genomes(KEGG)enrichment analyses obesity-related DEGs were performed using DAVID software and R software.The data were visualized using Cytoscape software,and hub genes were calculated by cytoHubba tool.Quantitative real-time PCR(qRT-PCR)was used to detect the expression levels of hub genes in posterior longitudinal ligament tissue.Results:Twenty-four OPLL obesity-related DEGs were obtained by screening GSE69787 and obesity-related genes.A protein-protein interaction network of these 24 obesity-related DEGs was constructed and calculated,and finally,10 important hub genes were selected.GO/KEGG enrichment analyses indicated that these 10 obesity-related DEGs were mainly enriched in biological processes and signaling pathways such as protein kinase B signaling pathway and its regulation,ossification,regulation of inflammatory response,bone mineralization,PI3K-Akt signaling pathway and MAPK signaling pathway.Finally,qRT-PCR verified that LEP,IGF2,IL18,BMP2,and LOX were highly expressed in OPLL ligament tissue(P＜0.05),while MMP9 and PTGS2 were low expressed in OPLL ligament tissue(P＜0.05).Conclusions:LEP,IGF2,IL18,BMP2,LOX,MMP9,and PTGS2 are genes associated with obesity in OPLL patients.The abnormal expression of these genes may be related to the occurrence and development of OPLL,providing new ideas for the diagnosis and treatment of OPLL patients with obesity.
Cervical VertebraOssification of Posterior Longitudinal LigamentObesityGene
万方数据
维普
