Objective:To investigate the efficacy and accuracy of the anterior pedicle screw placed assisted by O-arm navigation.Methods:From January 2020 to December 2021,20 patients with atlantoaxial dislocation who underwent transoral atlantoaxial reduction plate were retrospectively analyzed.They were divided into a navigation group and a freehand group acording to the screw placement methods.The operation time,intraopeative blood loss,preoperative and postoperative clivus-canal angle(CCA)and cervico-medullary angle(CMA)were recorded for the evaluation of clinical efficacy.The accuracy of pedicle screw placement was evaluated according to the postoperative cervical CT and improved screw grading criteria proposed by modified Park standard.Results:There was no significant difference in the operation time,intraopeative blood loss,preoperative or postoperative CCA or CMA between the two groups(P>0.05).Postoperative CCA and CMA were significantly larger than preoperative ones in both groups(P<0.05).The accuracy of screw placement in the navigation group was significantly higher than that in the freehand group(P<0.05).Conclusions:Anterior pedicle screw placement assisted by O-arm navigation can achieve the same clinical efficacy with the freehand screw placement.Meanwhile,it can improve the accuracy and safety of screw placement and reduce postoperative complications.