核酸适配体的应用现状及其在骨科领域的应用研究进展
Research progress of nucleic acid aptamers and their application in orthopedics
杨钱冬 1周江玲 1杨明 1侯天勇1
作者信息
- 1. 中国人民解放军陆军军医大学附属第一医院骨科,重庆 400038
- 折叠
摘要
核酸适配体是经指数富集的配体系统进化(SELEX)技术筛选得到的单链寡核苷酸分子序列.因其与作用物具有较高的亲和力与特异性,较传统的抗体具有成本更低、稳定性更高、无免疫原性等优点,目前已被广泛应用于食品安全、环境监测、医学检验、靶向药物等多种领域.当前,其在骨科还是一个新兴的应用与研究领域,主要进展体现在骨与软骨组织再生、骨肿瘤疾病的诊治.因核酸适配体筛选流程繁琐、失败率高,目前关于其的研究与应用较少.本文对核酸适配体的应用现状及其在骨科领域的应用研究进展进行综述.
Abstract
Nucleic acid aptamers refer to single-stranded oligonucleotide molecular sequences obtained through systematic evolution of ligands by exponential enrichment(SELEX)technology.Due to their high affinity and specificity,nucleic acid aptamers offer advantages such as lower cost,higher stability and no immunogenicity compared to traditional antibody techniques.They are currently widely used in various fields,including food safety,environmental monitoring,medical diagnostics,and targeted drug delivery.In orthopedics,nucleic acid aptamers represent a new application and research field with main progress focused on the regeneration of bone and cartilage tissue,and the diagnosis and treatment of bone tumor diseases.However,there is currently limited research and application of nucleic acid aptamers due to the complicated selection process and high failure rate of nucleic acid aptamers.This article reviews the current application of nucleic acid aptamers and their research progress in the field of orthopedics.
关键词
核酸适配体/指数富集的配体系统进化技术/骨与软骨组织再生/骨肿瘤Key words
Aptamer/Systematic Evolution of Ligands by Exponential Enrichment Technology/Regeneration of Bone and Cartilage Tissue/Bone Tumor引用本文复制引用
基金项目
重庆市技术创新与应用发展专项重点项目(CSTB2023TIAD-KPX0008)
重庆市自然科学基金面上项目(CSTB2023NSCQ-MSX0426)
出版年
2024
NETL
NSTL
万方数据