Anti-infection performance of aminomethyl blue modified poly glycidyl methacrylate modified titanium implant in orthopedics
Objective:To evaluate the anti-infection performance of aminomethyl blue modified poly glycidyl methacrylate modified titanium implant(Ti-PGMB).Methods:A fluorescent probe was used to confirm the production of reactive oxygen species(ROS)by Ti-PGMB implant under light irradiation.To further evaluate the antibacterial effectiveness of Ti-PGMB,MRSA biofilm models and animal models with implant-related infections were established.Mice were randomly divided into three groups:Ti implant control(Ti),Ti-PGMB without light irradiation(Ti-PGMB-L),and Ti-PGMB with light irradiation(Ti-PGMB+L),with 10 mice in each group.In the Ti group,titanium tablets were implanted,while the Ti-PGMB was implanted in the Ti-PGMB+L and Ti-PGMB-L groups.MRSA bacterial suspensions(5×107 CFU/mL)were inoculated at the implantation sites.After 24 hours,the Ti-PGMB+L group received continuous light intervention for 7 days,while no additional interventions were performed in the Ti and Ti-PGMB-L groups.The recovery of the incisions at the implant site and the number of MRSA in the tissue homogenates near the implant were assessed on days 3 and 7 after intervention.The levels of inflammatory factors in tissue homogenates were measured by enzyme linked immunosorbent assay,and tissue inflammation was assessed by hematoxylin-eosin staining.Results:Under light irradiation,Ti-PGMB implants can generate ROS and dissipate mature biofilms.In vivo anti-infection experiments revealed the survival rates of bacteria in the Ti-PGMB-L group were 97.7%and 98.9%at days 3 and 7 after intervention,respectively,which was comparable to the Ti group(100%)(all P>0.05).In contrast,the survival rates of bacteria were 47.1%and 7.0%in the Ti-PGMB+L group at days 3 and 7 after intervention,respectively,which were statistically significantly lower compared to the Ti group(100%)(all P<0.05).At day 7 after intervention,the levels of the inflammatory factors interleukin-1β,interleukin-6,and tumor necrosis factor-α in tissue homogenates of the Ti-PGMB+L group were significantly lower than those in the Ti-PGMB-L and Ti groups(all P<0.05).Conclusions:Ti-PGMB,through its controllable photodynamic effect,can generate ROS that sterilize infectious bacteria and their biofilms.Ti-PGMB has significant clinical value in inhibiting implant-related infections represented by MRSA and reducing inflammations.
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