工业污染区PM2.5对HEK293细胞表达hERG钾通道和Nav1.5钠通道的影响
Effect of PM2.5 from industrial areas on expression of potassium channel hERG and sodium channel Nav1.5 in HEK293 cells
赵慧君 1牛俊杰 1王娟娟 1陈鸿 1匡兴亚1
作者信息
- 1. 同济大学附属杨浦医院职业病科,上海 200090
- 折叠
摘要
目的 研究工业污染区PM2.5对去极化Nav1.5钠离子通道和复极化hERG钾离子通道的影响.方法 采集工业污染区和非污染城区的空气制备PM2.5样本,采用气相色谱质谱联用仪(GC-MS)和电感耦合等离子体质谱(ICP-MS)分析其中的多环芳烃类、金属离子和无机盐离子等主要成分,采用Nav1.5-HEK293细胞和hERG-HEK293细胞分别测试10、30、100、300、1 000 μg/ml浓度时PM2.5的抑制作用.结果 在10～1 000μg/ml浓度时工业污染区的PM2.5样本对hERG钾离子通道呈浓度依赖性的抑制作用;工业污染区和非污染城区的PM2.5对Nav1.5钠离子通道均有明显的抑制作用,半数抑制浓度(IC50)分别为90和200 μg/ml.结论 PM2.5对心肌细胞钠、钾离子通道的抑制作用可能是其心脏毒性作用机制之一.
Abstract
Objective Study the effects of PM2.5 from industrial polluted areas on the depolarization of Nav1.5 sodium ion channel and the repolarization of hERG potassium ion channel.Methods The air samples from polluted industrial areas and non-polluted urban areas to prepare PM2.5 samples,analyze the main components such as polycyclic aromatic hydrocarbons,metal ions,and inorganic salt ions using gas chromatography-mass spectrometry(GC-MS)and inductively coupled plasma mass spectrometry(ICP-MS),test the inhibitory effects of PM2 5 at concentrations of 10,30,100,300,and 1 000 μg/ml,using Nav1.5-HEK293 cells and hERG-HEK293 cells,respectively.Results The results showed that at the concentration of PM2.5 between 10～1 000 μg/ml,the samples from polluted industrial area exhibited concentration dependent inhibitory effect on hERG potassium ion channels;while the PM2.5 samples both from industrial polluted area and the non-polluted urban area exhibited significant inhibitory effects,with the half-maximal inhibitory concentrations(IC50)of 90 and 200 µg/ml,respectively.Conclusion The results suggested that the inhibitory effect of PM2.5 on sodium and potassium ion channels may be one of its mechanisms of cardiotoxicity.
关键词
PM2.5/心脏毒性/hERG钾离子通道/Nav1.5钠离子通道Key words
particulate matter 2.5 μm(PM2.5)/cardiotoxicity/hERG potassium ion channel/Nav1.5 sodium ion channel引用本文复制引用
出版年
2024
NETL
NSTL
万方数据