摘要
目的 观察经皮穴位电刺激(transcutaneous electrical acupoint stimulation,TEAS)对去势后SD大鼠血清骨代谢指标及骨密度(bone mineral density,BMD)的影响.方法 大鼠随机分为Sham组(假手术组)、0VX 组(模型对照组)、2 Hz组(0VX+TEAS 2 Hz组)、50 Hz组(0VX+TEAS 50 Hz组),每组8 只,3 个月后分别检测血清骨代谢指标骨特异性碱性磷酸酶(BALP)、Ⅰ型原胶原C端前肽(PICP)、Ⅰ型原胶原N端前肽(PINP)、Ⅰ型胶原羧基端肽(ICTP)、吡啶啉(Pyr)、脱氧吡啶啉(D-Pyr)及BMD.结果 0VX组较Sham组血清BALP、PICP、PINP、ICTP、Pyr、D-Pyr均显著增高(P<0.05);与OVX组比较,2 Hz组血清BALP、PINP、PICP、ICTP、Pyr、D-Pyr均显著降低,腰椎、右股骨BMD增加(P<0.05),50 Hz组血清BALP、PINP、PICP 及BMD无明显变化,ICTP、Pyr、D-Pyr亦均显著降低(P<0.05);与 50 Hz组比较,2 Hz组腰椎BMD增高(P<0.05).结论 经皮穴位电刺激可能通过调控骨吸收和骨形成指标,参与抗骨质疏松作用,且 2Hz电刺激频率可能较 50 Hz更适用于临床骨质疏松症的防治.
Abstract
Objective To observe the effects of transcutaneous electrical acupoint stimulation(TEAS)on serum bone metabolic indexes and bone mineral density(BMD)in ovariectomized SD rats.Methods Thirty-two rats were randomly divided into Sham group(sham operation group),OVX group(model control group),2 Hz group(OVX+TEAS 2 Hz group),and 50 Hz group(OVX+TEAS 50 Hz group),with 8 rats in each group.Serum bone metabolic indexes,including bone specific alkaline phosphatase(BALP),type Ⅰ procollagen C-terminal propeptide(PICP),type Ⅰ procollagen N-terminal propeptide(PINP),type Ⅰ collagen carboxyl-terminal peptide(ICTP),pyridinoline(Pyr),deoxypyridinoline(D-Pyr),and BMD were detected 3 months later.Results Compared to those in Sham group,serum BALP,PINP,PICP,ICTP,Pyr,and D-Pyr in OVX group increased significantly(P<0.05).Compared to those in OVX group,serum BALP,PINP,PICP,ICTP,Pyr,and D-Pyr in 2 Hz group decreased significantly.BMD in lumbar spine and right femur increased(P<0.05).BALP,PINP,PICP,and BMD in 50 Hz group had no significant changes.ICTP,Pyr,and D-Pyr also decreased significantly(P<0.05).Compared to that in the 50 Hz group,the lumbar BMD in the 2 Hz group was higher(P<0.05).Conclusion TEAS play a role in anti-osteoporosis,probably by regulating bone resorption and bone formation indexes.The electrical stimulation with frequency of 2 Hz may be more suitable for clinical prevention and treatment of osteoporosis than with 50 Hz.
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维普
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