Objective To explore the correlation between serum hepcidin concentration and bone density in the population,and to understand the effect of hepcidin on the differentiation of mouse osteoblast precursor cells(MC3T3-E1)and its related mechanisms.Methods ①Enzyme linked immunosorbent assay(ELISA)was used to detect serum hepcidin levels in two groups of people with normal bone mass and osteoporosis;② Using cell counting reagents to detect and compare the effects of osteogenic precursor cells(MC3T3-E1)on cell proliferation after intervention with different concentrations of hepcidin RT-PCR and Western Blot method were used to investigate the effects of hepcidin on osteogenic differentiation functional indicators(ALP,Runx2,BMP2,P-SMAD1/5,and SMAD5).Results There was a significant positive correlation between serum hepcidin levels and bone mineral density of the lumbar spine and hip in the population;An increase in the concentration of hepcidin within a certain range(0-10 ng/mL)can significantly upregulate the expression of cell functional differentiation(ALP,Runx2)and promote the expression of osteogenic signaling pathway proteins(BMP2,P-SMAD1/5,and SMAD5).Conclusion Clinical data shows a significant correlation between serum hepcidin levels and bone mass,with low hepcidin levels and low bone density values;Cell experiments have shown that a certain concentration of hepcidin can significantly activate the BMP2-SMAD1/5 signaling pathway,upregulate the expression of osteogenic differentiation related genes,and induce cell osteogenic differentiation;Therefore,this study suggests that serum hepcidin may be correlated with osteoporosis,and clinical detection of hepcidin may be a new evaluation indicator for the diagnosis and treatment of osteoporosis,which is worth further research.
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