Objective To investigate the effect of plastrum testudinis extract(PTE)in alleviation of bone loss and hippocampal degeneration in senile osteoporosis(SOP)mice by inhibiting cellular senescence.Methods Animal experiments:the traditional galactose subcutaneous injection+denervation method was applied to establish a SOP model.C57/BL6 mice were divided into female or male CON group,SOP group,and SOP+PTE group.Mice in SOP+PTE group was intervened with PTE 0.5 g/ml by gavage for 4 weeks during the modelling process.Behavioural experiments were carried out 1 week before sampling.The brain and L4 vertebrae were collected to detect bone and hippocampal histomorphology.Cellular experiments:MC3T3/HT22 cell senescence was induced by using tert-butyl peroxyhydroxypropanol(TBHP).The changes in HT22 galactosidase staining,senescence markers,and MC3T3 osteogenesis,senescence markers and the corresponding therapeutic effect of PTE were observed respectively after the induction.Results Micro CT result suggested that in both male and female mice,bone mass was lost in the SOP group compared to that in the CON group,while bone mass was restored in the SOP+PTE group.The result of bone HE section were in line with those of micro CT.Results in hippocampal tissue section suggested that hippocampal degeneration was observed in the SOP group compared to that in the CON group,while the degeneration was alleviated in the SOP+PTE group.In the new object recognition experiment and temporal sequence experiment,the memory ability of the male SOP+PTE group was significantly restored compared to that in the CON and SOP groups.However,there was no significant change in the absent field experiment in both male and female mice.② TBHP induced MC3T3 senescence,decreased osteogenic capacity,and increased senescent protein expression.When PTE was added,it reversed the decrease in osteogenic capacity and protein decline caused by TBHP.TBHP also increased the HT22 galactosidase staining region and increased senescent protein expression.When PTE was added,the above changes were reversed.Conclusion PTE alleviates bone loss and hippocampal senescence in SOP mice by inhibiting the senescence of preosteoblasts and hippocampal neuronal cells.
关键词
龟板提取物/细胞衰老/老年性骨质疏松/骨量丢失/海马组织退变
Key words
plastrum testudinis extract/cell senescence/senile osteoporosis/loss of bone mass/hippocampal degeneration
维普
万方数据