Objective To examine the result of mechanical vibration on the expression of neuropeptide calcitonin gene-related peptide(CGRP)and substance P(SP)during fracture healing in rats with osteoporotic fracture after ovarian detotyping.Methods Thirty 3-month-old female Wistar rats were randomly assigned to control group(Sham),ovariectomy group(OVX)and vibration group(OVX-V),with 10 rats in each group.The ovaries of the rats in the ovariecto-ovary group and the vibration group were excised to establish an osteoporosis model,and the control group was excised with the same mass of adipose tissue to establish a pseudo-ovarian-departed osteoporosis model,and the rats in the vibration group were subjected to vibration intervention with a frequency of 35Hz,an amplitude of 2 mm,and an acceleration of 0.5 g for 20 min,and the other groups were placed on a closed shaking table for 20 min and moved freely for 5 days per week.After 2 and 6 weeks of intervention,X-ray films were taken to observe and compare the healing of the three groups.The expressions of CGRP and SP at the fracture end were detected by enzyma-linked immunosorbent assay(ELISA).Results The fracture healing rate of the Sham group and the OVX-V group was higher than that of the OVX group after 2 weeks of intervention,and the fracture healing rate of the OVX-V group was higher than that of the other two groups after 6 weeks of intervention(P＜0.05).After 2 weeks of intervention,the CGRP content[0.464±0.018]and SP content[0.450±0.019]in the bone tissue of rats in the OVX-V group were higher than those in the OVX group but significantly lower thanthoseinthe Sham group(P＜0.05).After6weeksofintervention,the CGRPcontent[0.632±0.016]and SP content[0.636±0.017]in the bone tissue of rats in the OVX-V group showed an upward trend and was greatly higher than that in the OVX group,but still lower than that in the Sham group(P＜0.05).Conclusion Mechanical vibration helps to increase the expression levels of neuropeptides CGRP and SP in the bone tissue of rats with osteoporotic fractures,thereby increasing osteoblast activity and inhibiting bone resorption and accelerating fracture healing.
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