摘要
目的 分析司美格鲁肽对糖尿病性骨质疏松(diabetic osteoporosis,DOP)大鼠骨代谢及炎症因子的调节机制.方法 选择 45 只SD大鼠,随机分为空白组、模型组、司美格鲁肽组 3 组,每组各 15 只.比较 3 组大鼠的血清FBG、FINS、炎症因子、骨密度、骨代谢指标、p-PI3K、PI3K、pAkt、Akt蛋白水平、生物力学相关参数及观察其股骨组织HE染色、股骨组织TRAP染色、脂肪组织HE染色.结果 空白组大鼠脂肪细胞大小均匀、结构清晰,且结构较为完整,炎性细胞浸润显著减少.与空白组比较,模型组的破骨细胞/骨表面的平均数量、TRAP、OC、Ca、ALP、P、FBG、TNF-α、IL-6 显著升高(P<0.05);与模型组比较,司美格鲁肽组的破骨细胞/骨表面的平均数量、TRAP、OC、Ca、ALP、P、FBG、FINS、TNF-α、IL-6显著降低(P<0.05).与空白组比较,模型组的骨密度、FINS、p-PI3K/PI3K、p-Akt/Akt、股骨弹性模量、最大应力、最大载荷、弹性载荷显著降低(P<0.05);与模型组比较,司美格鲁肽组的骨密度、FINS、p-PI3K/PI3K、p-Akt/Akt、股骨弹性模量、最大应力、最大载荷、弹性载荷显著升高(P<0.05).结论 司美格鲁肽可有效改善DOP大鼠的骨代谢及炎症反应,其作用机制可能与激活PI3K/Akt通路相关.
Abstract
Objective To analyze the regulatory mechanism of semaglutide on bone metabolism and inflammatory factors in diabetic osteoporosis(DOP)rats.Methods Forty-five SD rats were randomly divided into 3 groups,blank group,model group and semaglutide group,with 15 rats in each group.The serum FBG,FINS,inflammatory factors,bone mineral density,bone metabolism indexes,p-PI3 K,PI3 K,pAkt,Akt protein levels,biomechanical related parameters,HE staining of femoral tissue,TRAP staining of femoral tissue,and HE staining of adipose tissue were compared among the three groups.Results The size of adipocytes in the blank group was uniform,the structure was clear,and the structure was relatively complete,but the infiltration of inflammatory cells was significantly reduced.Compared to those in the blank group,the average number of osteoclasts/bone surface,TRAP,OC,Ca,ALP,P,FBG,TNF-α,and IL-6 in the model group increased significantly(P<0.05).Compared to those in the model group,the average number of osteoclasts/bone surface,TRAP,OC,Ca,ALP,P,FBG,FINS,TNF-α,and IL-6 in the smeglutide group decreased significantly(P<0.05).Compared to those in the blank group,bone mineral density,FINS,p-PI3K/PI3K,p-Akt/Akt,femoral elastic modulus,maximum stress,maximum load,and elastic load of the model group reduced significantly(P<0.05).Compared to those in the model group,bone mineral density,FINS,p-PI3K/PI3K,p-Akt/Akt,femoral elastic modulus,maximum stress,maximum load,and elastic load of the semaglutide group increased significantly(P<0.05).Conclusion Semaglutide effectively improves bone metabolism and inflammatory response in DOP rats.Its mechanism may be related to the activation of PI3K/Akt pathway.
基金项目
国家自然科学基金面上项目(82274544)
江西省中医药管理局科技计划项目(2023B0280)
广州市科技计划项目(202206010184)
2021年广东省教育厅普通高校重点领域专项(2021ZDZX2005)
NETL
NSTL
万方数据