Objective To study whether oral sheep placenta powder canprevent postmenopausal osteoporosis.Methods Established an osteoporosis model and randomly divide it into sham surgery group(Sham),model group(OVX),sheep fetal disc powder low(L),medium(M),high-dose group(H),and ethinylestradiol group(EE).The whole body bone density is measured every two weeks.After 1.5 months,weighed and calculated the main organ coefficients,detected the in vitro BMD and serum bone metabolism indexes,and performed Micro-CT scanning to observe the changes of bone tissue morphology.Results After 6 weeks,the overall bone density of the OVX group rats was significantly lower than that of the Sham group(P＜0.01),but there was no significant decrease in the EE group.The three groups of sheep fetal discs were significantly higher than those of the OVX group(P＜0.01).The changes in bone mineral density of the isolated femur showed a similar trend,but there was no significant difference between the Low group and OVX(P＞0.05).Micro-CT scan analysis showed that among the three groups of sheep placenta,only the parameters of group M weresimilar to those of Sham group and EE group,and HE staining of histomatological sections of femur after decalcification showed similar result to those of Micro-CT.The three-point bending test of the femur found that the maximum load and strength of the M group were significantly higher than those of the OVX group(P＜0.01,P＜0.05).In serum bone metabolism indicators,the bone formation indicators BAP and PINP in the M group were significantly higher than those in the OVX group,while the bone resorption indicators CTX-I and TRACP were significantly lower than those in the OVX group.During the experiment,there were no significant differences in body weight among all groups,no obvious abnormalities in the appearance of the heart,liver,spleen,lung,kidney and other major organs,no significant differences in organ coefficients except the uterus of the ethoestradiol group,and no significant abnormalities were found in pathological examination except the uterus of the ethoestradiol group.Conclusion Oral administration of sheep placenta powder[300 mg/(kg·d)]can prevent osteoporosis in ovaries excised rats.The dual activities of promoting bone formation and inhibiting bone absorption may be an important mechanism of anti-osteoporosis,and sheep placenta powder has no side effect of thickening endometrium.
关键词
羊胎盘粉/切卵巢大鼠/骨密度/骨质疏松症/骨形成
Key words
sheep placenta powder/ovariectomized rats/bone mineral density/osteoporosis/bone formation
维普
万方数据