The Efficacy of a kidney-tonifying and spleen-strengthening prescription in mediating gastrointestinal homeostasis and mitigating bone loss in ovariectomized rats
The Efficacy of a kidney-tonifying and spleen-strengthening prescription in mediating gastrointestinal homeostasis and mitigating bone loss in ovariectomized rats
Objective To explore the mechanism of intestinal homeostasis in bone loss in ovariectomized(OVX)rats and the preventive and therapeutic effects of kidney tonifying and spleen strengthening formula.Methods A cohort of eighteen 4-month-old female Sprague-Dawley(SD)rats,which were nulliparous,were randomly assigned into three groups:a sham-operated control group(SHAM),an ovariectomized group(OVX),and a group treated with the kidney-tonifying and spleen-strengthening prescription(BSJP),with each group comprising six subjects.Following a 5-day post-operative recovery period,the SHAM and OVX groups were administered saline via gavage,whereas the BSJP group received the prescribed formulation via the same route for a duration of 12 weeks prior to sample collection.Analyses included Micro-CT imaging of the femur to evaluate bone density and microarchitecture,histological examination with hematoxylin and eosin(HE)staining of the small intestine and femur tissues,enumeration of osteoclasts through tartrate-resistant acid phosphatase(TRAP)staining,and Western Blot analyses to quantify the expression levels of the intestinal barrier protein Claudin-3(Cldn3),pro-inflammatory cytokines TNF-α and IL17A,chemokines CCL20 and CXCR3,and osteoclast-associated proteins C-FOS,DC-STAMP,and Cathepsin K(CTSK)in both the intestinal and bone tissues.Results Comparative analysis between the OVX and BSJP groups revealed a significant thickening of the intestinal wall,attenuation of damage to the villous architecture of the small intestine,and a reduction in osteoclastic activity within the femur in the BSJP group.Micro-CT scans demonstrated a notable increment in bone mineral density(BMD).Elevated expression of the intestinal barrier protein Cldn3 was observed in conjunction with a decrease in the levels of pro-inflammatory markers TNF-α,IL17A and chemokines CCL20,CXCR3 as well as a reduction in osteoclast-associated proteins C-FOS,DC-STAMP,and CTSK.Conclusion Osteoporosis in ovariectomized(OVX)rats stems from a dysregulation of gastrointestinal homeostasis.The administration of the kidney-tonifying and spleen-strengthening formula appears to restore this balance,thereby ameliorating the bone loss associated with OVX.
关键词
绝经后骨质疏松症/补肾健脾方/骨量丢失/肠道稳态
Key words
postmenopausal osteoporosis/kidney-tonifying and spleen-strengthening prescription/bone loss/gastrointestinal homeostasis
维普
万方数据