Comprehensive Comparison and Validation of eDNA Metabarcoding Primers for Phytoplankton Biomonitoring
Environmental DNA(eDNA)metabarcoding technology has high throughput and good repeatability,and has great application potential in the future ecological environment monitoring.At present,eDNA monitoring for phytoplankton is still in the development stage,and there is a lack of unified amplification primers.The differences of 8 pairs of universal primers in phytoplankton eDNA monitoring were comprehensive evaluated and compared with the same field environmental samples,which provided support for the initial establishment of standardized phytoplankton eDNA monitoring methods.The results showed that different primers had obvious preference for phytoplankton amplification.Primers targeting amplification of 16S rDNA mainly detected out Bacillariophyta,followed by Cryptophyta and Chlorophyta.The three pairs of primers 1391,AD3 and ANF,which targeted amplification of 18S rDNA,showed high amplification efficiency and species identification,and 67,62 and 63 genera of phytoplankton were detected,respectively.According to the relative abundances of sequences,ranking of the top five phytoplankton was Bacillariophyta>Chlorophyta>Cryptophyta>Chrysophyta>Dinophyta.Moreover,these three pairs of primers could be used as universal primers for phytoplankton eDNA metabarcoding monitoring.
NETL
NSTL
万方数据
