Direct Determination of 15 Kinds of Perfluoroalkyl Substances in Water by Liquid Chromatography-Tandem Mass Spectrometry Coupled with Online Solid Phase Extraction
A method of automated on-line solid phase extraction-liquid chromatography-tandem mass spectrometry(on-line SPE LC-MS/MS)to analyze 15 kinds of perfluoroalkyl substances(PFASs)in water was developed by optimizing the conditions of sample saving,pre-treatment and analysis,and the PFASs in drinking water source and peripheral water were quantitatively detected.After passing through the membrane filter,a 700 μl sample was moved into a 1.5 mL autosampler vials with 300 μL methanol.A 800 μl sample was directly injected into the online SPE flow path.Online purification and enrichment of the samples were carried out on the SPE column(Bio WAX NP3,4 mm×10 mm,3 μm).The PFASs were eluted from the SPE column and then transferred to the analytical flow path by valve switching.PFASs were separated on HPH-C18 column(2.1 mm×50 mm,2.7 μm),and further analyzed by mass spectrometer detector,and quantified by using isotope internal standard method.The total analysis time of the method was 22 min.The detection limits of PFASs were in 0.31-1.20 ng/L.The average recoveries for 15 PFASs at low,medium and high spiked levels ranged from 70%to 115%,with relative standard deviations(RSD,n=6)of 0.2%-14.0%.PFASs concentrations in the real samples analyzed by this method and conventional method were close to each other,but the proposed method was simple,rapid,high automation and solvent-saving.The established method was sensitive and accurate,and suitable for detection of 15 kinds of PFASs in surface water.
perfluoroalkyl substances(PFASs)surface watertap wateron-line solid phase extraction(on-line SPE)liquid chromatography tandem mass spectrometry
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