Research on the mechanism of Berberine inhibiting the formation of Klebsiella pneumoniae biofilm
Objective To investigate the effect of Berberine on the biofilm formation of Klebsiella pneumoniae(KP),and provide theoretical basis for the diagnosis and treatment of KP related infections.Methods The effect of Berberine on KP biofilm formation was observed by crystal violet semi-quantitative method.The expression of typeⅠ and type Ⅲ FIMi structural genes fimA,fimH,mrkA,mrkD and regulatory genes fimK and mrkH in KP detected by RT-PCR at 1/8MIC,1/4MIC and 1/2MIC doses.Results Compared with the control group,the total amount of biofilm in berberine group decreased significantly with increasing concentration,and the difference was statistically significant(P＜0.05).Compared with the control group,the expression levels of fimA,fimH,mrkA,mrkD,fimK and mrkH genes in berberine group were significantly decreased(P＜0.05).Conclusion Berberine effectively inhibits KP biofilm formation by down-regulating typeⅠand typeⅢFIMi structural genes fimA,fimH,mrkA,mrkD and regulatory genes fimK and mrkH.
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