Zinc transporter 9 inhibiting prostatitis induced by high-fat food in mice
Objective To investigate whether zinc inhibit prostatic inflammation induced by high-fat food and explore its mechanism.Methods 3-week-old C57BL/6 male mice were randomly divided into 3 groups:normal food group,high-fat food group and high-fat food + zinc group(zinc sulfate gavage),with 10 mice in each group.All mice were killed after feeding for 10 weeks.The weight and prostatic weight of mice were weighed and the prostatic index was calculated.HE staining was used to detect the infiltration of inflammatory cells in prostate tissue.The expression of proinflammatory factors,IL-6,TNF-α and zinc transporter-9(ZnT-9)were detected by immunohistochemistry.Results Compared with the normal food group,body weight(P<0.001),prostatic weight(P<0.001)and prostatic index(P<0.01)of mice in the high-fat food group were significantly increased,and the prostatic weight(P<0.01)and prostatic index(P<0.05)of mice in the high-fat food + zinc group were significantly lower than those in the high-fat food group.The inflammatory cell infiltration in the prostate tissue of mice in the high-fat food group was significantly increased than that in the normal food group,and the inflammatory cell infiltration in the prostate tissue of mice in the high-fat food+zinc group was significantly reduced than that in the high-fat food group.Compared with the normal food group,the expression of IL-6 and TNF-α was significantly increased(P<0.05)and the expression of ZnT-9(P<0.01)was significantly decreased in the high-fat food group.Compared with the high-fat food group,the expression of IL-6 and TNF-α were significantly reduced(P<0.05),and the expression of ZnT-9(P<0.05)was significantly increased in the high-fat food + zinc group.Conclusion Zinc inhibits high-fat food-induced prostatitis in mice,and the mechanism may be related to the upregulation of ZnT-9.
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