Analysis of expression status of HER2,clinicopathological characteristics and prognostic in breast cancer
Objective To explore the relationship between HER2 status,clinicopathological characteristics and prognosis in patients with invasive breast cancer.Methods Selected 629 invasive breast cancer patients who had undergone HER2 IHC and FISH detection at the same time in Fuzhou University Affiliated Provincial Hospital,and analyzed HER2 status,clinicopathological characteristics and prognosis using the 2019 version of breast cancer HER2 detection guidelines.Results IHC results showed that 0～1+,2+,3+accounted for 53.10%,22.26%,24.64%respectively;The negative and positive cases of FISH results accounted for 66.67%and 33.23%.There was a statistically significant difference in FISH detection results among different tumor types(P=0.001).There was no significant difference in the detection results of the two methods between different gender,age of onset,tumor size,histological grade and lymph node metastasis(P>0.05).For female cases,the difference between IHC and FISH was statistically significant(P<0.001);Identify the consistency test between negative and positive cases,Kappa=0.925,P=0.000;Correlation analysis,r=0.696,P=0.000.The follow-up case survival curve showed that the DFS was higher in the Herceptin-treated group than that in the non-treated group,and the difference in IHC negative FISH positive patients was significant(P<0.05).There was no significant difference between consistent and discordant cases.Conclusion Based on the 2019 version of breast cancer HER2 detection guidelines,FISH detection results are related to tumor type.The results of IHC and FISH are in good agreement,and the two results are positively correlated.The detection rate of FISH method is higher than that of IHC method.When conditions permit,it is recommended to do FISH detection for confirmation.When the results are inconsistent,FISH results may have more guiding significance for clinical treatment decisions.
Invasive breast cancerHuman epidermal growth factor receptor 2Fluorescence in situ hybridizationImmunohistochemistry
万方数据
维普
