The migration and invasion of giloma cells promoted by SERINC2 through activating the TGF-β/Smad3 signaling pathway
Objective To investigate the effects of serine incorporated 2(SERINC2)on the migration and invasion of glioma cells and its related molecular mechanisms.Methods The GEPIA database was used to analyze the differential expression of SERINC2 mRNA between glioma tissue and normal brain tissue.CGGA database was used to detect the relationship between the expression level of SERINC2 mRNA and the survival rate of glioma patients.shRNA was used to construct U251 cell lines knockdown of SERINC2.qRT-PCR and Western blot were used to detect knockdown efficiency.The effects of SERINC2 on the migration and invasion ability of U251 cells were detected by scratch assay and Transwell assay,respectively.Western blot was used to detect the expression of epithelial mesenchymal transition(EMT)and TGF-β/Smad3 pathway related proteins.Results The expression of SERINC2 mRNA in glioma tissue was higher than that in normal brain tissue,and the 10-year survival rate of patients with high expression of SERINC2 mRNA was significantly lower than that of patients with low expression(P<0.05);Compared with the control group,the migration and invasion ability of U251 cells decreased after knockdown SERINC2(P<0.05),and the expression level of E-cadherin protein increased,while the expression levels of TGF-β,Smad2/Smad3,p-Smad3 and N-cadherin proteins decreased.Conclusion SERINC2 is highly expressed in gliomas.It affects the EMT of glioma cells by activating the TGF-β/Smad3 signaling pathway and promotes the migration and invasion of glioma cells.
NETL
NSTL
万方数据
