Effects of repeated inhalation of sevoflurane during neonatal period on hippocampal ApoE and learning and memory in rats
Objective To investigate the effects of repeated inhalation of sevoflurane anesthesia on hippocampal ApoE and learning and memory in rats during neonatal period.Methods Forty-eight clean healthy SD rats(7 days old),half male and half female,weighed 15～20g,were randomly divided into control group(Air group,n=24)and sevoflurane anesthesia group(Sev group,n=24).Rats in Sev group inhaled 2.6%sevoflurane+carrier gas(1L/min air+1L/min O2)for 2 hours at 7 day,14 day and 21 day after birth,while rats in Air group only inhaled carrier gas(1L/min air+1L/min O2)for 2 hours.Each group was divided into two subgroups according to the time of specimen collection:the newborn group(P21,21 days after birth)and the adult group(P97,97 days after birth).Morris water maze test was performed at 91～97 days after birth to test the learning and memory ability.Brain tissue was taken(samples were taken at 2 hours after inhalation of sevoflurane on the 21st day in neonatal group;in the adult group,samples were taken after Morris water maze test).The expression of ApoE in hippocampus was detected by immunohistochemistry.ApoE gene expression was detected by qRT-PCR.Results Compared with Air group,the expression of ApoE and ApoE mRNA in hippocampus of neonatal rats increased(P<0.05).There was no significant change in ApoE and ApoE gene expression in different hippocampal regions of adult rats(P>0.05).There was no significant difference in the indexes of Morris water maze between two groups in adulthood(P>0.05).Conclusion Repeated inhalation of sevoflurane during neonatal period can up-regulate the expression of ApoE in the hippocampus of rats in neonatal period and produce transient reversible neurotoxicity,but it has no effect on learning and memory function of rats in adulthood.
Neonatal periodSevofluraneInhalation anesthesiaApolipoprotein ELearning and memory
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