首页|Aptamer-Based Antibiotic Electrochemical Detection Platform Using Portable Plastic Gold Electrode and Multichannel Chip
Aptamer-Based Antibiotic Electrochemical Detection Platform Using Portable Plastic Gold Electrode and Multichannel Chip
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The development of accurate,rapid and practical electrochemical sensing technology for antibiotic detection has an important ap-plication prospect in many fields such as food safety,environmental monitoring,medical and health care.In this research,kanamycin specific DNA aptamer and its partially complementary short chain were self-assembled onto the surface of an integrated portable plastic gold electrode(PGE)by Au-S bond,and a simple kanamycin electrochemical biosensor interface was constructed.At the same time,the epoxy resin channel layer was designed,and the auxiliary instrument was set up independently in the laboratory,which could help to measure and analyze eight groups of samples automatically in turn.The quantitative analysis results showed that in 20 min,the sensor had a good linear relationship between the peak current change of square wave voltammetry and the negative logarithm of kanamycin concentration when kanamycin concentration was in the range of 1-1000 μmol/L,and the detec-tion limit could reach 0.40 μmol/L.In addition,the discrimination could be achieved within 5 min in the real-time analysis mode.The sensor had a simple construction method,good selectivity and stability,and could be used for rapid or real-time detection of kana-mycin residues in actual water samples,which provided a new direction for the practical detection of kanamycin in environmental water samples.
BiosensorAptamerAntibioticsElectroanalysisDNA
Yin Bao、Kaiwei Cai、Yichen Liu、Bingling Li
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Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun,Jilin 130022,China
School of Applied Chemistry and Engineering,University of Science and Technology of China,Hefei,Anhui 230026,China
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