首页|Immunological Exploration of Helicobacter pylori Serotype O2 O-Antigen by Using a Synthetic Glycan Library
Immunological Exploration of Helicobacter pylori Serotype O2 O-Antigen by Using a Synthetic Glycan Library
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Helicobacter pylori(H.pylori)infection is a threat to human health.The lipopolysaccharide(LPS)O-antigen holds promise for devel-oping vaccines.It is meaningful to explore the immunological activity of oligosaccharides with different lengths and frameshifts for antigen development.Herein,a glycan library of H.pylori O2 O-antigen containing eight fragments is constructed.After screening with anti-H.pylori O2 LPS sera and patients'sera by glycan microarray,the disaccharide HPO2G-2b and trisaccharide HPO2G-3a show strong antigenicity and then are separately conjugated with carrier protein CRM197.Both glycoconjugates elicit a robust im-munoglobulin G(IgG)immune response in rabbits.The anti-HPO2G-3a IgG antibodies possess a much stronger binding affinity with the LPS and bacteria of H.pylori O2 than the anti-HPO2G-2b IgG antibodies.There is no cross-reaction between both sera IgG anti-bodies with LPS and bacteria of H.pylori O1,O6,and E.coli.The results demonstrate the trisaccharide HPO2G-3a is a promising can-didate for H.pylori vaccine development.
Ming Zhao、Guangzong Tian、Chunjun Qin、Xiaopeng Zou、Peter H.Seeberger、Jing Hu、Jian Yin
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Key Laboratory of Carbohydrate Chemistry and Biotechnology,Ministry of Education,School of Biotechnology & School of Life Sciences and Health Engineering,Jiangnan University,Lihu Avenue 1800,Wuxi,Jiangsu 214122,China
Biomolecular Systems Department,Max Planck Institute of Colloids and Interfaces,Am Mühlenberg 1,Potsdam,14476,Germany
Wuxi School of Medicine,Jiangnan University,Lihu Avenue 1800,Wuxi,Jiangsu 214122,China
国家自然科学基金国家自然科学基金国家自然科学基金国家自然科学基金国家自然科学基金中国博士后科学基金高等学校学科创新引智计划(111计划)Max Planck Society International Partner Group ProgramChina Scholarship Council(CSC)for fundingMax Plank Society for generous financial support
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