首页|A High-Contrast Autolysosome Probe for Detecting Interaction between Autophagosomes and Autolysosomes in Mitophagy
A High-Contrast Autolysosome Probe for Detecting Interaction between Autophagosomes and Autolysosomes in Mitophagy
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NETL
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Autophagy is a multi-step cell metabolism process in which cells remove damaged and unwanted materials.During autophagy,au-tophagosomes fuse with lysosomes to form autophagosomes.Autophagosomal membrane components are recycled from autolyso-somes through the autophagosomal components recycling(ACR),while lysosomal components circulate on the autolysosomal sur-face through the autophagic lysosome reformation(ALR)process.Autolysosomes contain components from autophagosomes and lysosomes.However,whether there is a fusion between autolysosome and autophagosome or lysosome at the organelle level re-mains unknown.In this study,a pH and viscosity dual-controlled mitochondria-targeting fluorescent probe Mito-Q was designed based on an asymmetric norcyanine to achieve the high-contrast imaging of mitochondria-containing autolysosomes.Mito-Q not only effectively detected mitochondrial viscosity changes and mitophagy with high sensitivity,but more importantly,the fusion of mitochondria-containing autolysosomes and autophagosomes(FMAA)was observed during autophagy by the real-time confocal im-aging of HeLa cells.
NETL
NSTL
万方数据
