首页|Direct Analysis of Whole Blood by a Disposable Monolithic Column Mass Spectrometry Analysis Kit
Direct Analysis of Whole Blood by a Disposable Monolithic Column Mass Spectrometry Analysis Kit
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万方数据
维普
A monolithic column-based mass spectrometry(MS)analysis kit was prepared for whole blood analysis with MS.The kit is disposable and can be used for purification,storage,transportation and direct analysis of whole blood.The kit mainly consists of a capillary for quantitative microsampling,a cation exchange monolithic column for purification and storage,and a syringe for loading sample.This kit is very friendly to various users that one can easily siphon the blood in the kit followed by rapid clean-up.We established a quanti-tative method using the kit with a limit detection as low as 0.33 nmol/L,and achieved more than five orders of magnitude enhance-ment in sensitivity compared to direct nanoelectrospray ionization MS analysis.The column can avoid analyte exposure to environ-ment,which helps the storage of the sample for laboratory analysis.The relative standard deviation of immediate blood analysis and storage blood analysis within 10 d was less than 10％.This method has been successfully applied to the quantitative analysis of pro-cainamide hydrochloride in 2 μL rat blood.These results indicate that this disposable kit does have the potential to achieve highly sen-sitive quantitative MS analysis in biological samples,which is expected to become a cost-effective and powerful tool for in vitro diag-nostics.
万方数据
维普
