首页|An Efficient Probe for Bacterial Nitroreductase Imaging and Detection Based on NanoLuc-Furimazine Bioluminescent Pair

An Efficient Probe for Bacterial Nitroreductase Imaging and Detection Based on NanoLuc-Furimazine Bioluminescent Pair

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The detection of critical endogenous species,such as bacteria in microenvironments in the body,requires better imaging tools for visualization and monitoring of biological events.Bioluminescence imaging is the most popular strategy for obtaining real-time in living cells and organisms.Herein,we introduced a nitroaryl group on the C-3 position and a hydroxy group at the C-6 phenyl ring on furimazine to report the first bioluminescent probe(7)based on NanoLuc-furimazine bioluminescent pair for the detection of ni-troreductase in bacteria.The probe,which possessed up to 560-fold intensity increase with a low detection limit of 16 ng/mL of ni-troreductase,has the most efficient uncage efficiency in comparison with other bioluminescent congeners,thus enabling highly se-lective and sensitive visualization of NTR activity in a panel of clinical priority pathogens.Additionally,imaging of the recombinant strain as well as the NTR from mouse feces indicated the potential of this probe in the application of different mouse disease mod-els.

NitroreductaseNanoLuc-furimazineBiosensorsAnalytical methodsLuminescenceImaging agents

Ximeng Shi、Yumeng Wang、Jiaoyang Yu、Yating Yang、Zecheng Jin、Hanghang Wang、Dalei Wu、Wei Chen、Jianming Guo、Yinan Zhang

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Jiangsu Key Laboratory for Functional Substances of Chinese Medicine,School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing,Jiangsu 210023,China

Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing,Jiangsu 210023,China

Clinical Research Center,the Second Hospital of Nanjing,Affiliated Hospital to Nanjing University of Chinese Medicine,Nanjing,Jiangsu 210003,China

Key Laboratory of Resources Biology and Biotechnology in Western China,Ministry of Education,College of Life Sciences,Northwest University,Xi'an,Shaanxi 710069,China

Helmholtz International Lab,State Key Laboratory of Microbial Technology,Shandong University,Qingdao,Shandong 266237,China

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Program of the National Natural Science Foundation of ChinaProgram of the National Natural Science Foundation of ChinaProgram of the National Natural Science Foundation of ChinaJoint Training Program for Graduated Students of the Institute of Translational Research and Drug Innovation

221770528207413682272340

2024

10.1002/cjoc.202300702

中国化学(英文版)
中国化学会 上海有机化学研究所

中国化学(英文版)

CSTPCD
影响因子：0.848
ISSN：1001-604X
年,卷(期)：2024.42(12)