首页|Regulating CRISPR/Cas9 Using Streptavidin-Biotin Interactions

Regulating CRISPR/Cas9 Using Streptavidin-Biotin Interactions

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Currently,CRISPR/Cas9 technology has found widespread applications across various domains.However,the utility of CRISPR/Cas9 is encumbered by issues pertaining to its reliability and safety,primarily stemming from the uncontrolled activity of the system.There-fore,the design and development of CRISPR/Cas9 systems with controllable activity is of paramount importance.Biotin,characterized by its small molecular weight,and streptavidin,distinguished by its substantial spatial steric hindrance,can be harnessed as an ideal OFF switch(termed a"bioactivity brake")due to their interaction characteristics.In this work,we present a strategy that employs the streptavidin-biotin interaction as a"brake system"for CRISPR/Cas9,effectively allowing for the shutdown of the enzymatic activity of CRISPR/Cas9.

Streptavidin-biotinCRISPR/Cas9CRISPR-OFFGuide RNA2'-OH acylationGene technologyDNA cleavage

Wei Shen、Wei Xiong、Qianqian Qi、Xingyu Liu、Zhongpao Xie、Yuanyuan Zhang、Jinxuan Hou、Tian Tian、Xiang Zhou

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College of Chemistry and Molecular Sciences,Wuhan University,Wuhan,Hubei 430072,China

Department of Thyroid & Breast Surgery,Zhongnan Hospital of Wuhan University,Wuhan,Hubei 430071,China

National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaFundamental Research Funds for the Central UniversitiesTranslational Medicine and Interdisciplinary Research Joint Fund of Zhongnan Hospital of Wuhan University

22177089217210059215330322037004221770882042023kf0204ZNJC202309

2024

10.1002/cjoc.202300662

中国化学(英文版)
中国化学会 上海有机化学研究所

中国化学(英文版)

CSTPCD
影响因子：0.848
ISSN：1001-604X
年,卷(期)：2024.42(12)