首页|High Mannose-Specific Aptamers for Broad-Spectrum Virus Inhibition and Cancer Targeting
High Mannose-Specific Aptamers for Broad-Spectrum Virus Inhibition and Cancer Targeting
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NETL
NSTL
维普
万方数据
High mannose oligosaccharides are characteristic and essential for immune evasion of many viruses and cancer cells.They are potential targets for viral inhibition and cancer diagnosis/therapy.Particularly,high mannose-binding reagents may be a unique asset for fighting the ongoing and mutating SARS-CoV-2 virus.Lectins are prevailing reagents for saccharide binding but suffer from inadequate spec-ificity and apparent immunogenicity.Meanwhile,oth-er reagents for the same purpose,such as antibodies and aptamers,have rarely been reported.Herein,using molecularly imprinted magnetic nanoparticles as a potent platform,we report a smart selection method for fine screening of high mannose-specific aptamers.Monovalent aptamers were first effective-ly screened within eight rounds of selection.Multiva-lent aptamers,in the forms of dendritic polymer or tetrahedral DNA nanostructure(TDN),were further engineered.The aptamers exhibited high affinity to-ward the spike protein of SARS-CoV-2 and the enve-lope protein GP120 of HIV.Both the monovalent aptamer and its TDN form exhibited a certain inhibition effect to the SARS-CoV-2 pseudovirus.On the other hand,both the monovalent aptamer and its dendritic form permitted the recognition of cancer cells over normal cells.Therefore,as unprecedented reagents for broad-spectrum viral inhibition and can-cer targeting,these aptamers hold great promise for clinical treatment and diagnosis.
NETL
NSTL
维普
万方数据
