Enzyme-and catalyst-generated reactive species have been leveraged in the past decade to covalently label biomolecules within a short range of a defined site or space inside cells or at the cell-cell interface.Due to their high spatial resolution,such proximity labeling strategies have been coupled with various bioanalytical techniques for dissecting dynamic and complex biological processes.Here,we review the development of enzyme-and catalyst-triggered proximity chemistry and their applications to identi-fying protein interaction networks as well as cell-cell communications in living systems.
proximity labelingbioorthogonal chem-istryspatiotemporal proteomicscell-cell interac-tionsliving systems
Yanjun Liu、Yun Ge、Ruxin Zeng、William Shu Ching Ngai、Xinyuan Fan、Peng R.Chen
展开 >
Synthetic and Functional Biomolecules Center,Beijing National Laboratory for Molecular Sciences,Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education,College of Chemistry and Molecular Engineering,Peking University,Beijing 100871
National Key Laboratory of Crop Genetic Improvement,Hubei Hongshan Laboratory,Huazhong Agricultural University,Wuhan 430070
Shenzhen Bay Laboratory,Institute of Chemical Biology,Shenzhen 518107
NETL
NSTL
万方数据
维普
