首页|Effect of carbon source on growth and lipid accumulation in Chlorella sorokiniana GXNN01

Effect of carbon source on growth and lipid accumulation in Chlorella sorokiniana GXNN01

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Heterotrophic culture of microalgae to develop methods of increasing biomass productivity and storage lipids has brought new insight to commercial biodiesel production. To understand the relationship between heterotrophy and lipid production, the effects of carbon sources on the growth and lipid accumulation of Chlorella sorokiniana GXNN01 was studied. The alga exhibited an increased growth rate in response to the addition of carbon sources, which reached the stationary phase after 48 h at 30°C. In addition, glucose and NaAc had a significant effect on the lipid accumulation during the early-stationary phase. Specifically, the lipid content was 0.237±0.026 g g~(-1) cell dry weight and 0.272±0.041 g L~(-1) when glucose was used as the carbon source, whereas the lipid content reached 0.287±0.018 g g~(-1) cell dry weight and 0.288±0.008 g L~(-1) when NaAc was used as the carbon source. The neutral lipid content was found to first decrease and then increase over time during the growth phase. A glucose concentration of 20 mmol L~(-1) gave the maximal lipid yield and the optimum harvest time was the early-stationary phase.

heterotrophicmicroalgaebiodieselcarbon sourcelipid

QIAO Hongjin、WANG Guangce

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Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China

Graduate School,Chinese Academy of Sciences,Beijing 100039,China

Tianjin University of Science & Technology,Tianjin 300222,China

国家高技术研究发展计划(863计划)Key Project of Science and Technology for Supporting Tianjin DevelopmentKnowledge Innovation Project of Chinese Academy of Sciences

2006AA05Z1122007LS700310KGCX2-YW-374-3

2009

10.1007/s00343-009-9216-x

海洋湖沼学报(英文版)
中国海洋湖沼学会

海洋湖沼学报(英文版)

CSTPCDCSCDSCI
影响因子：0.386
ISSN：2096-5508
年,卷(期)：2009.27(4)
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