首页|Oyster studies reveal the duplication and functional diversification of Bivalvia caspase-8 genes
Oyster studies reveal the duplication and functional diversification of Bivalvia caspase-8 genes
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Caspase-8,first classified as a pro-apoptotic caspase,is considered to have arisen from duplication with caspase-10 and involves multiple immune and inflammatory responses in mammals.However,few are known on the phylogeny and function of caspase-8 in molluscs,one of the largest phyla in marine invertebrates.In this study,we conducted phylogenetic and functional analysis on molluscan caspase-8-like genes.Results indicate that duplication occurred in molluscan caspase-8-like genes,resulting in at least two caspase-8 copies in some groups of bivalves.Additional studies in Pacific oyster Crassostrea gigas showed different spatio-temporal expression patterns and subcellular localizations of CgCaspase-8-1 and CgCaspase-8-2.While no interaction was observed between CgCaspase-8-2 and CgFADD,the adaptor molecule in apoptosis,yeast two-hybrid and co-immunoprecipitation assays suggested the interaction between CgCaspase-8-1 and CgFADD,indicating its pro-apoptotic function.In addition,CgCaspase-8-1 showed interaction with the CARD domain of CgRIG-I.Together with two NF-κB subunits(Cgp105 and CgRel),their transcripts were up-regulated in response to poly(I:C)stimuli,supporting the immune function of both pro-and anti-inflammation.The results provide insight into the evolution and functional diversification of Bivalvia caspase-8 genes.
CAS and Shandong Province Key Laboratory of Experimental Marine Biology,Center for Ocean Mega-Science,Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China
University of Chinese Academy of Sciences,Beijing 100049,China
NHC Key Laboratory of Diagnosis and Therapy of Gastrointestinal Tumor,Gansu Provincial Hospital,Lanzhou 730000,China
Laboratory for Marine Biology and Biotechnology,Qingdao National Laboratory for Marine Science and Technology,Qingdao 266000,China
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Science & Technology Innovation Project of Laoshan LaboratoryCenter for Ocean Mega-Research of Science,Chinese Academy of SciencesGHfund BTaishan Scholars Program
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