首页|Proteomic response of Phaeocystis globosa to nitrogen limitation

Proteomic response of Phaeocystis globosa to nitrogen limitation

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  • 国家科技期刊平台
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Phaeocystis globosa is an important unicellular eukaryotic alga that can also form colonies.P.globosa can cause massive harmful algal blooms and plays an important role in the global carbon or sulfur cycling.Thus far,the ecophysiology of P.globosa has been investigated by numerous studies.However,the proteomic response of P.globosa to nitrogen depletion remains largely unknown.We compared four protein preparation methods of P.globosa for two-dimensional electrophoresis(2-DE)(Urea/Triton X-100 with trichloroacetic acid(TCA)/acetone precipitation;TCA/acetone precipitation;Radio Immuno Precipitation Assay(RIPA)with TCA/acetone precipitation;and Tris buffer).Results show that the combination of RIPA with TCA/acetone precipitation had a clear gel background and showed the best protein spot separation effect,based on which the proteomic response to nitrogen depletion was studied using 2-DE.In addition,we identified six differentially expressed proteins whose relative abundance increased or decreased more than 1.5-fold(P<0.05).Most proteins could not be identified,which might be attributed to the lack of genomic sequences of P.globosa.Under nitrogen limitation,replication protein-like,RNA ligase,and sn-glycerol-3-phosphate dehydrogenase were reduced,which may decrease the DNA replication level and ATP production in P.globosa cells.The increase of endonuclease Ⅲ and transcriptional regulator enzyme may affect the metabolic and antioxidant function of P.globosa cells and induce cell apoptosis.These findings provide a basis for further proteomic study of P.globosa and the optimization of protein preparation methods of marine microalgae.

Phaeocystis globosanitrogen limitationproteomic responsetwo-dimensional electrophoresis

Haisu LIU、Ruiwang WEI、Qiangyong LEI、Lei CUI、Songhui Lü

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Key Laboratory of Eutrophication and Red Tide Prevention,Research Center for Harmful Algae and Marine Biology,Jinan University,Guangzhou 510632,China

National Engineering Laboratory of Electronic and Information Standardization,Shenzhen 518057,China

Southern Marine Science and Engineering Guangdong Laboratory,Zhuhai 519082,China

National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaMarine Economic Development Project of Guangdong ProvinceBasic and Applied Basic Research Project of GuangzhouBasic and Applied Basic Research Project of GuangzhouOutstanding Innovative Talents Cultivation Funded Programs for Doctoral Students of Jinan University

4217614241906111418061272023B11110500112023A04J15482023A04J15492021CXB010

2024

10.1007/s00343-023-2313-4

海洋湖沼学报(英文版)
中国海洋湖沼学会

海洋湖沼学报(英文版)

CSTPCD
影响因子：0.386
ISSN：2096-5508
年,卷(期)：2024.42(1)
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