首页|Effects of pyraclostrobin on growth,oxidative stress,and gene expression in relation to stress and ATP-binding cassette transporters in Tetrahymena thermophila

Effects of pyraclostrobin on growth,oxidative stress,and gene expression in relation to stress and ATP-binding cassette transporters in Tetrahymena thermophila

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Pyraclostrobin(PYR),a widely used fungicide,has negative effects on fish and algae,but its toxicity in protozoa remains unclear.In this study,the effects of PYR on the growth,oxidative stress,and gene expression related to stress and ATP-binding cassette(ABC)transporters in Tetrahymena thermophila were investigated.The result showed that the 96-h IC50 of PYR against T.thermophila was 17.2 mg/L.Moreover,PYR inhibited the growth of T.thermophila in concentration-or time-dependent manner.A morphological study revealed that the shape and size of T.thermophila changed,and damage of cell membrane surface was observed by scanning electron microscopy after 96 h of PYR exposure.The activities of superoxide dismutase(SOD)and catalase(CAT)increased throughout the experiment.In contrast,the glutathione(GSH)content was increased at 24 h and 48 h of exposure and decreased at 96 h.Moreover,a significant increase in malondialdehyde(MDA)level was observed in T.thermophila after 96 h of exposure.Furthermore,PYR upregulated the HSP703,HSP705,GPx2,and ABAC15 gene expression in the 0.1-5-mg/L groups and downregulated the HSP704,HSP90,TGR,and ABCC52 mRNA levels at 96 h of exposure.These results suggest that PYR may exert adverse effects on T.thermophila by inducing oxidative stress and changing the gene expression related to ABC transporters and stress,which may enrich the understanding of the toxicity mechanism of PYR in aquatic organisms and provide reference data for aquatic ecological risk assessments.

pyraclostrobinTetrahymena thermophilagrowthoxidative stressgene expression

Yang LIU、Jiale ZHANG、Peng XIAO、Xin LIU、Yisifu MA、Jing ZHANG、Bangjun ZHANG

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Henan International Joint Laboratory of Aquatic Ecotoxicology and Health Protection,Xinxiang 453007,China

College of Life Sciences,Henan Normal University,Xinxiang 453007,China

Journal of Henan Normal University,Xinxiang 453007,China

College of Life and Environmental Science,Wenzhou University,Wenzhou 325035,China

Department of Ecosystem Studies,School of Environmental Science,University of Shiga Prefecture,Shiga 522-8533,Japan

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Key Scientific Research Projects of Henan Province to College Youth Backbone TeacherNational Key Research and Development Program of China

20211182021YFE0112000

2024

10.1007/s00343-023-2378-0

海洋湖沼学报(英文版)
中国海洋湖沼学会

海洋湖沼学报(英文版)

CSTPCD
影响因子：0.386
ISSN：2096-5508
年,卷(期)：2024.42(1)
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