首页|Analysis of environmental selection pressure of superoxide dismutase in deep-sea sea cucumber
Analysis of environmental selection pressure of superoxide dismutase in deep-sea sea cucumber
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
国家科技期刊平台
NETL
NSTL
万方数据
Manganese superoxide dismutase(MnSOD)is an antioxidant that exists in mitochondria and can effectively remove superoxide anions in mitochondria.In a dark,high-pressure,and low-temperature deep-sea environment,MnSOD is essential for the survival of sea cucumbers.Six MnSODs were identified from the transcriptomes of deep and shallow-sea sea cucumbers.To explore their environmental adaptation mechanism,we conducted environmental selection pressure analysis through the branching site model of PAML software.We obtained night positive selection sites,and two of them were significant(97F→H,134K→V):97F→H located in a highly conservative characteristic sequence,and its polarity change might have a great impact on the function of MnSOD;134K→V had a change in piezophilic ability,which might help MnSOD adapt to the environment of high hydrostatic pressure in the deepsea.To further study the effect of these two positive selection sites on MnSOD,we predicted the point mutations of F97H and K134V on shallow-sea sea cucumber by using MAESTROweb and PyMOL.Results show that 97F→H,134K→V might improve MnSOD's efficiency of scavenging superoxide anion and its ability to resist high hydrostatic pressure by moderately reducing its stability.The above results indicated that MnSODs of deep-sea sea cucumber adapted to deep-sea environments through their amino acid changes in polarity,piezophilic behavior,and local stability.This study revealed the correlation between MnSOD and extreme environment,and will help improve our understanding of the organism's adaptation mechanisms in deep sea.
Yanan LI、Zongfu CHEN、Haibin ZHANG、Ruoyu LIU、Shuichun CHEN、Li LIN
展开 >
Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding,College of Animal Sciences and Technology,Zhongkai University of Agriculture and Engineering,Guangzhou 510222,China
Institute of Deep-sea Science and Engineering,Chinese Academy of Sciences,Sanya 572000,China
College of Marine Science,Fujian Agriculture and Forestry University,Fuzhou 350002,China
Zhanjiang Tianli Sea Cucumber New Technology Development Co.,Ltd.,Zhanjiang 524000,China
展开 >
Guangdong Province Basic and Applied Basic Research Fund Project国家自然科学基金Major Scientific and Technological Projects of Hainan Province
国家科技期刊平台
NSTL
万方数据
