首页|Molecular cloning and functional analysis of two calcium-associated cuticular protein genes in Neocaridina denticulata sinensis

Molecular cloning and functional analysis of two calcium-associated cuticular protein genes in Neocaridina denticulata sinensis

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  • 国家科技期刊平台
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  • NSTL
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The body surface of crustaceans is covered with a sturdy shell.The growth and development of crustaceans are realized through molting.Neocaridina denticulata sinensis is a suitable candidate for crustacean scientific research.Two calcium-associated cuticular protein genes,named NdCAP-1 and NdCAP-2,were obtained from N.denticulata sinensis.Molecular docking simulated the binding effect of both proteins and calcium ions.Semi-quantitative reverse transcription PCR results show that NdCAP-1 is expressed in D2-4 stage,NdCAP-2 expressed inD24and A-B stages,and both were significantly expressed in the cephalothorax cuticle and pereiopods.Then,it was revealed that NdCAP-1 and NdCAP-2 are regulated by NdEcR-mediated 20E signaling pathways.Knockdown of NdCAP-1 and NdCAP-2 was observed to cause surface defects.The recombinant proteins(rNdCAP-1 and rNdCAP-2),obtained by prokaryotic expression,had calcium-binding and chitin-binding ability,inhibited formation of calcium carbonate precipitate.These results show that calcium-associated cuticular proteins play important roles in cuticle formation and calcification.

Neocaridina denticulata sinensiscalcium-associated cuticular proteinmolting cycleRNAicalcium-binding ability

Xi GAO、Mengmeng DUAN、Yuying SUN、Jiquan ZHANG

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School of Life Sciences/Hebei Basic Science Center for Biotic Interaction,Hebei University,Baoding 071002,China

Institute of Life Science and Green Development,Hebei University,Baoding 071002,China

National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaKey Research and Development Project of Hebei ProvinceScience and Technology Project of Hebei Education DepartmentNatural Science Foundation of Hebei ProvinceNatural Science Foundation of Hebei ProvinceHangzhou Qianjiang Special Expert fund for Prof.Jiquan ZHANG

321729543237312122323201DZD2022093D2022201003D2023201002

2024

10.1007/s00343-024-3211-0

海洋湖沼学报(英文版)
中国海洋湖沼学会

海洋湖沼学报(英文版)

CSTPCD
影响因子：0.386
ISSN：2096-5508
年,卷(期)：2024.42(5)